In 1975, antiquarian Fausto Casi began collecting the more than 1000 apparatuses that document the history and evolution of means of communication. The collection includes period radios, from the Marconi patent to 1940’s radios, and historical material on cinema, from nineteenth-century optical games to the amateur cinema of the 1960s. There is also a collection of scientific instruments tied to the discovery of X-rays, such as old vacuum pumps and Geissler and Roentgen tubes. The period pieces are joined by historical-bibliographical material: a rich library, archive documents, film footage and photographic documents.

Housed in Palazzo Sabatini, since December 2005 the collection has become the permanent exhibition "Per un museo dei mezzi di comunicazione. Suono - Scrittura - Immagine" ["For a museum of communications. Sound - Writing - Images"], managed by "The World at Home" cultural association.

Situated in the Palazzo del Marzocco, the Centre illustrates the Battle of Anghiari (1440), made famous by the fresco, now lost, that the Florentines commissioned Leonardo da Vinci to paint in commemoration of their victory. The Commune of Anghiari has designed a special permanent exhibition area to display a replica on canvas of Leonardo's work, commissioned of Vittoria Chierici, along with a relief model and a number of multimedia posts for the virtual reconstruction of the battle. Leonardo's fresco is also documented by anastatic copies of his drawings, as well as a series of wedding chests dating from the third quarter of the 15th century.

Sections dedicated to the weapons used in the Battle of Anghiari, to military engineering and to local history are now being set up.

In 1564 the Company of the Holy Spirit was founded in Anghiari; it later took the name of the Confraternita di Misericordia, dedicated to caring for the sick and burying the dead. In 1870 a Misericordia Hospital was also founded. Starting in 1975, the Historical Archives have been rearranged and the Museum located in the ancient building of Badia, the Brotherhood’s historic residence, has been renovated.

The collection displayed in the Museum consists of various kinds of equipment for ambulances and for assisting the ill at home, dating from the late 19th century; uniforms of the Anghiari Misericordia and four means of transporting patients (a litter mounted on wheels from 1861, a small wagon-stretcher from 1909 called "emergency" for its use in emergencies, a wagon-structure on wheels to be pulled by hand, and an iron device for lifting patients from their beds).

The Museum, located in Palazzo Bourbon del Monte, was instituted in 2002 at the initiative of the Aboca company, active for over twenty years in the sector of medicinal plants, with the aim of providing historic evidence of the utilisation of herbs and reviving their traditional usage. Displayed in its exhibition rooms are antique utensils employed in ancient pharmacies to preserve and prepare medicinal herbs: mortars made of bronze, stone, alabaster, ivory and wood, of Italian and European provenance; majolicas, ceramics and blown glass jars. The material also includes tools for harvesting the herbs and laboratory instruments from the 18th century. Reconstructions of the traditional settings in which the herbs were prepared may also be seen.

The library contains printed books dating from the 16th through the early 20th century, relevant to botany, pharmacy and chemistry, as well as collections of botanical iconography and numerous herbariums (illustrated with copperplate engravings, woodcuts and lithographs).

Hosted in the church of San Giovanni Battista, the first news of which dates to 1126, the Museum of Stained Glass of Sansepolcro opened in 2003 and constitutes one of the first organic attempts in Italy to create a museum dedicated to stained glass. It mainly houses works that can be dated between the late 19th century and the early 20th century, including the outstanding life-size copy, in painted glass, of Leonardo’s Last Supper, made between 1937 and 1942 by Rosa and Cecilia Caselli and donated to the city by townsman Luigi Fatti. The Giuseppina Bernardini Collection instead contains stained glass with sacred subjects that can be dated to the second half of the 19th century, such as the large Crucifix, a masterpiece of British Pre-Raphaelite art.

Particularly interesting from a scientific viewpoint is the exhibition of stained glass samples (with and without figures), enriched by a collection of sketches, coloured panels and engravings from the late 18th century, which are joined by craftsmen’s workshop materials and texts illustrating the history, making and restoration of stained glass, and make it possible to trace back the phases of this ancient art. By appointment, demonstrations are offered on the making of traditional stained glass involving lead.